Endogenous calcineurin activity controls fin proximodistal regeneration. (A) PCE in the lof mutant regenerated (n = 5/6). (B) Fold change of calcineurin activities in lof mutant PCE or ACE normalized to control fins at 2 dpa (Student’s t test, *P < 0.05, ***P < 0.001). Data are presented as mean ± SD. (C) The expression of ppp3ca in the lof mutant at 2 dpa was lower compared with the control (Student’s t test, ***P < 0.001). Data are presented as mean ± SD. (D) Fold change of calcineurin activities in cav3 mutant regenerative fins normalized to control fins at 3 dpa (n = 7, Student’s t test, ***P < 0.001). Data are presented as mean ± SD. (E) Regeneration of the ACE was inhibited in cav3cq105 mutants (n = 8/8), while FK506 can rescue ACE regeneration at 7 dpa (n = 6/8) compared to WT (n = 7/8). White lines mark the amputation planes. Red lines show the extent of regenerative outgrowth, and black lines show the length of the entire uninjured caudal fin. (F) Quantification of the area of fin regenerative tissue (in square millimeters) (n = 5). (G) The regeneration of the PCE in cav3cq105 mutants was rescued by FK506 (n = 5/12) compared to control (n = 7/8). P, proximal; D, distal. (Scale bar: 500 µM in A and G; 2 mm in E.)
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