Expression of Uhrf1 in hsp70:Hmx1 transgenic zebrafish at 5 dpf.(A-D) Quantitative RT-PCR from RNA isolated from zebrafish at 5 dpf. (A) Expression of hmx1 in wildtype zebrafish without or with heat shock. (B) Expression of hmx1 in transgenic zebrafish without or with heat shock. (C) Expression of uhrf1 in wildtype zebrafish without or with heat shock. (D) Expression of uhrf1 in transgenic zebrafish without or with heat shock. Heat shock treatment induced expression of Hmx1 in hsp70:Hmx1 but not in wildtype zebrafish. Uhrf1 expression increased in hsp70:Hmx1 zebrafish embryos at 5dpf. (E) Lateral view of hsp70:Hmx1 zebrafish embryos at 5 dpf. In situ hybridization was performed with antisense RNA probe specific for uhrf1. Uhrf1 expression increased in response to ubiquitous hmx1 heat activation, but remained limited to the dorsal, eye and branchial arches regions. Bar, E 100 μm.
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