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Effect of cholangiocyte activation and inhibition on hepatocyte proliferation, apoptosis and fibrosis. 3-dpf kras + and WT zebrafish larvae were treated with either TCA or JTE-013 along with 20 µg/mL Dox till 8 dpf. Samples were collected for immunohistochemistry. kras+ and WT liver sections were incubated with primary antibodies for PCNA, Caspase 3a, Collagen or Laminin and then stained with Alexa Fluor 546 conjugated secondary antibody. All liver sections were counter-stained with DAPI. (A,C, E,G) Representative images of staining for PCNA (A), Caspase 3a (C), Collagen (E) and Laminin (G) of kras+ and WT zebrafish larvae in different groups. (B,D, F,H) Quantification of staining signals. For PCNA and Caspase 3a staining, number of stained cells were quantified. For Collagen and Laminin stainings, stained areas were quantified. N = 10 each group. Scale bar: 20 μm. Statistical significance: *P˂0.05.
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