FIGURE

Fig. 2.

ID

ZDB-FIG-210108-54

Publication

Carroll et al., 2020 - An Irf6-Esrp1/2 regulatory axis controls midface morphogenesis in vertebrates

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Fig. 2.

irf6,esrp1 and esrp2 are co-expressed in the oral epithelium of zebrafish embryos. (A-C) Whole-mount in situ hybridization (WISH), showing that irf6, esrp1 and esrp2 maternal deposited transcripts are detected at the eight-cell and shield stage (A; arrowheads indicate periderm), and circumscribe the developing stomodeum and line the oral epithelium of zebrafish embryos at 48 (B) and 72 (C) hpf (arrowheads). All whole-mount embryos are oriented with anterior left and dorsal top. (D-E″) Coronal sections of 48 (D) and 72 (E) hpf embryos analyzed by RNAscope in situ hybridization (ISH), (dorsal top), showing cellular RNA co-expression of irf6 (green) and esrp1 (white) in surface and oral epithelial cells. sox10 (red) staining depicts cartilage elements of the palate. Boxed areas are shown at higher magnification in D′, E′ and E″. Scale bars: 250 μm (A) and 100 μm (B-E″).

Expression Data

Genes:	esrp1 esrp2 irf6 sox10
Fish:	TU
Anatomical Terms:	epithelial cell EVL oral epithelium palate cartilage element stomodeum whole organism
Stage Range:	8-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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