FIGURE

Fig. 4

ID

ZDB-FIG-201113-25

Publication

Cook et al., 2020 - Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy

Other Figures

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Fig. 4

Ganglioside GM1 is increased in both SLOS and NPC zebrafish models. Representative images of cholera toxin subunit B (CtxB) staining of 96 hpf zebrafish to observe ganglioside GM1 distribution; a CtxB staining of SLOS zebrafish models induced using the Dhcr7 inhibitors AY9944 (75 µM) or trazodone (traz) (75 µM). Images of the zebrafish head from a lateral view (left) or dorsal view (right); b CtxB staining of NPC zebrafish models induced using either npc1 morpholino (npc1 MO) injected at the 1–2 cell stage or the Npc1 inhibitor, U18666A (2 µg/ml). Images of the zebrafish head from a dorsal viewpoint. Brain areas are indicated by coloured bars above images. Please note that the NPC images were taken using a separate Z.1 light sheet microscope that had been set up slightly differently. Scale bars = 280 µm. N = 3, with a minimum of ten fish per N

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL AB/TL + MO1-npc1
Conditions:	chemical treatment: N-[(2-chlorophenyl)methyl]-1-[4-[[(2-chlorophenyl)methylamino]methyl]cyclohexyl]methanamine chemical treatment: trazodone chemical treatment: amphiphile
Knockdown Reagent:	MO1-npc1
Observed In:	cerebellum ganglioside GM1 forebrain ganglioside GM1 hindbrain ganglioside GM1 midbrain ganglioside GM1
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Histochem. Cell Biol.