FIGURE

Figure 2—figure supplement 1.

ID

ZDB-FIG-201003-154

Publication

Weger et al., 2020 - MondoA regulates gene expression in cholesterol biosynthesis-associated pathways required for zebrafish epiboly

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Figure 2—figure supplement 1.

MO-mediated knockdown of <italic>chrebp</italic> and additional <italic>mondoa</italic> MO control experiments.

(A) Phenotypes of uninjected and water (CTR) injected embryos are indistinguishable from chrebp-mo injected embryos at bud stage (10 hpf). (B) Determination of the knockdown efﬁciency of the chrebp-mo. A reporter construct consisting of a part of the chrebp sequence around the transcription start site (+26/–34) cloned in front of a GFP reporter was co-injected with chrebp-mo or water (CTR) as a control. chrebp-mo injection efﬁciently abolished GFP expression in embryos. (C) Co-injection of an mRNA construct consisting of a part of the mondoa sequence around the transcription start site (−36/+20) cloned in front of a GFP reporter together with either mondoa-mis or mondoa-mo. mondoa-mo injection efﬁciently abolished GFP expression in embryos (n = 34), in contrast to mondoa-mis injection (n = 18). (D) Co-injection of a MO directed against p53 (p53–mo) and mondoa-mo (each 1 mM) did not alter the epiboly delay/arrest phenotype (n = 10/10, 100%). Abbreviations: BF, brightfield; GFP, GFP channel.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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