Assessment of llgl1 mutant gross morphology. (A) Diagram depicting the 10 bp deletion generated by TALENs in exon 1 of llgl1. (B) Embryos (3 dpf) from an llgl1+/−×llgl1−/− cross categorized into three grades of severity: class 0, wild-type phenotype; class 1 (mild), subtle signs of pericardial effusion at 3 dpf; class 2, pronounced pericardial effusion with a small subset displaying smaller eyes; and class 3 (severe), pronounced pericardial effusion and/or body edema, small eyes and hearts fail to undergo cardiac looping (Movie 5). At 5 dpf, llgl1−/− embryos are undistinguishable from llgl1+/− siblings. Scale bars: 1 mm. (C) Quantification of phenotype classes from llgl1+/−×llgl1−/− crosses comparing maternal contribution. Three clutches were used for each condition. n=109 for maternal llgl1−/− and n=160 for paternal llgl1−/−. χ2 test. (D) Adult genotyping from six pooled llgl1+/−×llgl1−/− crosses, greater than 4 months of age. The dashed line represents the predicted Mendelian number for each genotype. n=58 for llgl1+/− and n=31 for llgl1−/−. Two-tailed binomial test. Error bars indicate s.e.m. *P<0.05.
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