FIGURE

Fig. 5

ID

ZDB-FIG-200901-5

Publication

Izarzugaza et al., 2020 - Systems genetics analysis identifies calcium-signaling defects as novel cause of congenital heart disease

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Fig. 5

Functional validation of candidate genes in zebrafish. a Phenotype of controls and morphants. Single genes and combinations of genes targeted by splicing morpholinos are indicated on the left. Upper panels: gross appearance of 48 hpf zebrafish embryos. Lower panel: examples of cardiac phenotypes of 48 hpf wt, control, and morphant embryos. Hearts were visualized by ISH with a probe against the cardiac marker myl7. b Quantification of phenotypes in wt, controls, morphants, and mRNA rescued morphants. Note the combinatorial effects on cardiac phenotypes when more than one gene is affected. c, d Expression of mef2cb in 10 somite stage zebrafish embryos, analyzed by qRT-PCR (c) and ISH (d). ISH staining intensity was quantified and analyzed using Student’s T test. *p < 0.05, **p < 0.01

Expression Data

Gene:	mef2cb
Fish:	AB/TL + MO1-adcy2a AB/TL + MO1-itpr1b AB/TL + MO1-plcb2
Knockdown Reagents:	MO1-adcy2a MO1-itpr1b MO1-plcb2
Anatomical Terms:	anterior lateral plate mesoderm whole organism
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + MO1-adcy2a AB/TL + MO1-itpr1b AB/TL + MO1-plcb2 AB/TL + MO1-adcy2a + MO1-itpr1b AB/TL + MO1-adcy2a + MO1-plcb2 AB/TL + MO1-itpr1b + MO1-plcb2 AB/TL + MO1-adcy2a + MO1-itpr1b + MO1-plcb2
Knockdown Reagents:	MO1-adcy2a MO1-itpr1b MO1-plcb2
Observed In:	anterior lateral plate mesoderm atrioventricular canal development atrioventricular ring brain determination of heart left/right asymmetry heart heart looping post-vent region whole organism
Stage Range:	10-13 somites to Long-pec

Phenotype Detail

Acknowledgments

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