Figure 4

Neutrophilic response in CF hampers tissue repair in vivo. (A,B) Tail fin regeneration assessment in WT, cftr –/– and cftr MO zebrafish. Two dpf embryos were tail fin amputated then the potential of tail fin regeneration is evaluated by measuring regenerated fin areas at 3 dpi (blue). (A) Measurement of regenerated fin areas (n = 30, one-way ANOVA with Dunnett's post-test). (B) Representative imaging of injured tail fin at 3 dpi (scale bars, 200 μm). (C) Selective ablation of neutrophils has been carried out in WT and CF animals by injecting csf3r-MO. Measurement of regenerated fin areas in the presence or absence of neutrophils (n = 21, one-way ANOVA with Tukey post-test). (D)TgBAC(mpx:EGFP)i114 larvae were pretreated with DPI or H₂O₂ prior to tail fin amputation procedures, then injured and immediately put back in treatments for 4 h. Regenerated fin areas are measured at 3 dpi (n = 21, one-way ANOVA with Dunnett's post-test). (E) Inhibition of NADPH oxidase activity by injecting duox2-MO has been carried out in WT and CF animals. Embryos were tail fin injured and regenerated fin areas measured at 3 dpi (n = 21, two-tailed Bonferroni t-test).