FIGURE

Fig. 5

ID

ZDB-FIG-200813-13

Publication

Thiessen et al., 2019 - Zebrafish otolith biomineralization requires polyketide synthase

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Fig. 5

Aberrant expression of proteins involved in otolithdevelopment in csr and nco. (A) Schematic of anterior macula (AM) tethered to otolith at 27 hpf. (B) In WT, Otoconin-90 (Oc90) is expressed within the mineralized otolith, which is situated atop the otolithic membrane (Otogelin, or Otog), at 27 hpf. Scale bar = 5 μm. (C–D) Oc90 has diffuse expression within the otocyst of csr and nco. In csr and nco, Otog is localized near the apical surface of hair cells. (E–F) Expression showing hair cells in WT and nco larvae at 5dpf. Scale bar = 25 μm. (G) Quantification of hair cell numbers in the posterior and anterior macula of WT and nco(n = 4).

Expression Data

Antibodies:	AB1-oc90 Ab1-otof AB1-otog
Fish:	AB pks1^{cru101/cru101} (AB) pks1^jj149/jj149 (AB)
Anatomical Terms:	anterior macula otolith otolith organ posterior macula
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pks1^jj149/jj149 (AB)
Observed In:	hair cell anterior macula hair cell posterior macula
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 157, Thiessen, K.D., Grzegorski, S.J., Chin, Y., Higuchi, L., Wilkinson, C.J., Shavit, J.A., Kramer, K.L., Zebrafish otolith biomineralization requires polyketide synthase, 1-9, Copyright (2019) with permission from Elsevier. Full text @ Mech. Dev.