Fig. 3
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- ZDB-FIG-200709-53
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- Harris et al., 2020 - Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects
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Molecular Investigation of Novel Neuromuscular Synaptic Formation between Ectopically Directed CaP Neuron and Foreign Myotomes (A) Z projection of a zebrafish CaP neuron (red) deviated across a somitic boundary (dashed white line), revealed by α-bungarotoxin staining (cyan; scale bar, 10 μm). (B) A 3D rendering from confocal stacks of the deviated (red) and neighboring control (green) axon in (A) with the somitic boundary (blue; scale bar, 50 μm) (see also Video S3). (C) The axonal segments in the white dashed box in (A), pseudocolored to reflect Z position of the deviated CaP axon (white arrowheads), which passed lateral to the neighboring rostral CaP neuron (scale bar, 10 μm). (D) Same region as (C), showing the locations of the regions of interest in (E) on the unstimulated CaP axon (dashed white box, control) and the invading CaP axon (dashed white box, deviated; scale bar, 10 μm). (E) Immunohistochemistry of regions of interest in (D) for mCherry-PA-Rac1 (red), SV2 (green), and αBT (cyan) for the unstimulated control axon (top) and stimulated axon deviated across the somitic boundary (bottom). White arrowheads illustrate the colocalization of pre- and post-synaptic markers, suggesting the presence of synapses (n = 3 fish, Costes p value > 0.95; scale bar, 1 μm). R, rostral; C, caudal; D, dorsal; V, ventral. See also Figure S2, Video S3. |
Reprinted from Developmental Cell, 53, Harris, J.M., Wang, A.Y., Boulanger-Weill, J., Santoriello, C., Foianini, S., Lichtman, J.W., Zon, L.I., Arlotta, P., Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects, 577-588.e7, Copyright (2020) with permission from Elsevier. Full text @ Dev. Cell