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Cardiomyocyte numbers in <italic>MZjnk1a/b</italic> mutants and rescue of deficit with <italic>jnk1a</italic> transcripts.(A) Whole mount preparations of hearts from MZjnk1a/b mutants at completion of FHF addition (28hpf) and completion of SHF addition (50hpf). All cardiomyocytes are labelled wth mef2 antibody (red) and the atrial cardiomyocytes are labelled with atrial specific S46 antibody (green). Compared to wildtype (wt) embryos there is no reduction in ventricular size in MZjnk1b mutants at 28hpf or 50hpf. However the ventricular chamber is reduced in size in MZjnk1a null mutants at 28hpf and 50hpf. There is no further change in ventricular chamber size in MZjnk1a/MZjnk1b mutants. (B,D) Counting of ventricular cardiomyocytes confirms these findings. (C,E) There is no alteration in numbers of atrial cardiomyocytes. (F-J) Rescue of ventricular phenotype in (G,I) MZjnk1a and (H,J) MZjnk1a/MZjnk1b embryos with jnk1a transcripts at 28hpf (G,H) and 50hpf (I,J). Immunolabelling as in (A). The reduction in ventricular cardiomyocytes in MZjnk1a or MZjnk1a/MZjnk1b embryos is only reversed by injection of jnk1a Ex7 Lg transcript. ** = p<0.02, *** = p<0.001, **** = p<0.0001, ns = not significant.
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