FIGURE

Fig. S1

ID

ZDB-FIG-200513-18

Publication

Liu et al., 2020 - Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors

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Fig. S1

Mislocalized EYS in pomgnt1 mutant photoreceptors was not localized with mitochondria.
Cryosections of zebrafish eyes were double stained with anti-EYS (red) and with antibody against mitochondrial marker ATPB (green). The sections were counter-stained with DAPI to visualize nuclei (blue).

(A) Wild-type. In the wild-type retina, ATPB antibody labeled the ellipsoid region of photoreceptors with EYS immunoreactivity observed on the apical side of ATPB reactivity (arrows).
(B) Homozygous pomgnt1sny7 mutant. Most EYS immunoreactive puncta were localized in the outer nuclear layer. Some appeared to be over the ATPB immunoreactive domain (arrowheads)

(C-G) Mutant. Max projection image and its orthogonal views of EYS puncta that appeared to be over the ATPB immunoreactive domains. Of 81 EYS immunoreactive puncta that appeared to overlap with ATPB immunoreactivity, none were within the ATPB immunoreactive domain, indicating that mislocalized EYS were not within mitochondria.

Scale bar in A: = 4.36 m for A-B, 2 m for C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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