FIGURE

Figure 5

ID

ZDB-FIG-200406-183

Publication

Guillon et al., 2020 - Fibronectin is a smart adhesive that both influences and responds to the mechanics of early spinal column development

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Figure 5

Fibronectin is required for inter-tissue adhesion and a hsp70: fn1a-mKIKGR transgene rescues fn1a -/-; fn1b-/- double mutants.

(A–B) fn1a -/-; fn1b-/- double mutants exhibit a precociously converged neural tube and tissue detachment similar to MZ itgα5-/- mutants. (A) Transverse section 160 μm from last somite boundary in 12–14 somite stage fn1a-/-; fn1b -/- embryos with labeled nuclei (red) and F-actin (green). Dotted line delineates the neural tube. Arrowhead indicates tissue detachment. Scale bar = 70 μm. (B) Quantification of the medial-lateral length of the neural tube along the anterior-posterior axis starting from the last somite boundary (0 μm). Data represent means and SD. Immunostaining for Fibronectin (FN) on a 24 hpf heat shocked sibling (C), a non-heat shocked fn1a -/-; fn1b -/-; Tg hsp70: fn1a-mKIKGR embryo (D) and a heat shocked fn1a -/-; fn1b -/-; Tg hsp70: fn1a-mKIKGR embryo (E). Lateral views of the trunk with anterior to the left. DIC images of a heat shocked sibling (F), a non-heat shocked fn1a -/-; fn1b-/-; Tg hsp70: fn1a-mKIKGR embryo (G) and a heat shocked fn1a -/-; fn1b -/-; Tg hsp70: fn1a-mKIKGR embryo (H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fn1a^{ya13Tg/ya13Tg}; fn1b^{ya14Tg/ya14Tg} fn1a^{ya13Tg/ya13Tg}; fn1b^{ya14Tg/ya14Tg}; ya12Tg
Condition:	heat shock
Observed In:	posterior neural tube trunk Fibronectin
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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