FIGURE

Figure 5

ID

ZDB-FIG-200325-131

Publication

Katsouda et al., 2020 - Generation and Characterization of a CRISPR/Cas9 -Induced 3-mst Deficient Zebrafish

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Figure 5

Increased H₂O₂ production and oxidative stress sensitivity in 3-mst^−/− zebrafish. (A) Microscopic images show normal gross morphology of 3-mst^−/− larvae at 96 hpf in comparison with WT larvae. Black scale bar: 250 μΜ. (B) Mortality rate of WT and 3-mst^−/− larvae after PQ treatment. (C) Mortality rate of WT and 3-mst^−/− embryos after menadione (MN) treatment (D) Zebrafish H₂O₂ levels measured by Amplex Red Fluorescence in homogenates of WT and 3-mst^−/− at 96 hpf. Data are presented as mean ± SEM; n = 6–12/genotype; * P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mpst^aa102/aa102
Condition:	chemical treatment by environment: paraquat
Observed In:	cellular response to oxidative stress embryo development whole organism whole organism hydrogen peroxide
Stage Range:	Day 4 to Day 6

Phenotype Detail

Acknowledgments

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