FIGURE

Fig. 7

ID

ZDB-FIG-200302-20

Publication

Tu et al., 2019 - Isonitrile-responsive and bioorthogonally removable tetrazine protecting groups

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Fig. 7

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Fig. 7

Dual release of two orthogonal fluorophores from ICPr- and TzMe-caged dyes in live zebrafish. (a) Cartoon representation of experiment demonstrating the dual release and fluorescence turn-on of <italic>O</italic>-carboxymethyl fluorescein and resorufin upon reaction of <bold>4b</bold> and <bold>ICPr-rsf</bold>, and the corresponding control experiment with non-injected fish. (b) Visualization of fluorescein and resorufin fluorescence signal in live zebrafish (scale bar = 200 μm) injected with <bold>4b</bold> after a 2 h incubation with 10 μM <bold>ICPr-rsf</bold>. (c) Visualization of fluorescein and resorufin fluorescence signal in non-<bold>4b</bold> injected control live zebrafish (scale bar = 200 μm) after a 2 h incubation with 10 μM <bold>ICPr-rsf</bold>.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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