FIGURE

Fig 4

ID

ZDB-FIG-200220-33

Publication

Pálfy et al., 2020 - Chromatin accessibility established by Pou5f3, Sox19b and Nanog primes genes for activity during zebrafish genome activation

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Fig 4

Pou5f3, Sox19b and Nanog regulate chromatin accessibility at gene regulatory elements.

a) Volcano plots showing the log2 fold change in accessibility in MZpou5f3, MZsox19b and MZnanog mutants compared to wild-type embryos at oblong stage. Sites bound by Pou5f1, SoxB1, and Nanog are indicated in red, green and purple respectively. Non-bound sites are indicated in grey. b) Violin plots show the accessibility value (FPKM) at promoters and putative enhancers in wild-type embryos compared to the respective TF mutants. Significance of differences was tested using paired t-tests. c) Genome browser snapshots show accessibility tracks in MZpou5f3, MZsox19b and MZnanog mutants and wild-type embryos at oblong stage, for target genes of the respective TFs [15,25]. ChIP-seq tracks for the respective TFs at blastula stage are from data in [23,24]. d) Violin plots show the log2 fold change in mutants over wild-type at sites bound by different combinations of Pou5f3, SoxB1 and Nanog. p-values are shown for differences between binding sites as assessed by one-sided Wilcoxon tests.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nanog^m1435/m1435 pou5f3^{zf3267/zf3267} sox19b^{zf3268/zf3268}
Observed In:	chromatin remodeling
Stage:	Oblong

Phenotype Detail

Acknowledgments

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