FIGURE

FIGURE 6

ID

ZDB-FIG-200208-6

Publication

Erickson et al., 2020 - The lhfpl5 Ohnologs lhfpl5a and lhfpl5b Are Required for Mechanotransduction in Distinct Populations of Sensory Hair Cells in Zebrafish

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FIGURE 6

Lhfpl5a requires MET complex proteins Pcdh15a, Cdh23, and Myo7a for normal localization in the stereocilia of zebrafish hair cells. (A,B) Immunostain of Pcdh15a (magenta) in the lateral cristae of wild type and lhfpl5a^tm290d mutants at 3 dpf. Phalloidin-stained actin of the hair bundle is shown in green. Arrows indicate areas of Pcdh15a accumulation. (C–F’) Representative images of GFP-Lhfpl5a (vo23Tg) in the lateral cristae hair bundles of wild type (C,C’) and pcdh15a^psi7(D,D’), cdh23^nl9(E,E’), and myo7aa^ty220(F,F’) mutants. White arrows indicate GFP signal in the presumptive kinocilial linkages, yellow arrow heads indicate GFP signal in the stereocilia or the base of the hair bundle, and brackets indicate GFP signal in the kinocilium. Scale bars = 3 μm in (A,B); 2 μm in (C–F’).

Expression Data

Genes:	EGFP pcdh15a
Antibody:	Ab2-pcdh15a
Fish:	WT lhfpl5a^{tm290d/tm290d} vo23Tg cdh23^nl9/nl9; vo23Tg myo7aa^ty220/ty220; vo23Tg pcdh15a^psi7/psi7; vo23Tg
Anatomical Terms:	lateral crista kinocilium lateral crista stereocilium lateral crista stereocilium shaft lateral crista stereocilium tip
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lhfpl5a^{tm290d/tm290d} cdh23^nl9/nl9; vo23Tg myo7aa^ty220/ty220; vo23Tg pcdh15a^psi7/psi7; vo23Tg
Observed In:	lateral crista kinocilium lateral crista stereocilium lateral crista stereocilium bundle lateral crista stereocilium shaft lateral crista stereocilium tip
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.