Fig. 5
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- ZDB-FIG-200130-37
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- Dasyani et al., 2019 - Lineage tracing of col10a1 cells identifies distinct progenitor populations for osteoblasts and joint cells in the regenerating fin of medaka (Oryzias latipes)
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Contribution of col10a1 cells to the regenerating fin. A: Strategy employed for Gaudi (Cre-loxP)-mediated lineage tracing of col10a1 cells in the regenerating fin. Cre-induced recombination is triggered by incubating fish in 4-hydroxytamoxifen (4-HT) for three days starting at -5 dpa. B-C: Confocal images of GFP-labelled col10a1 cells in GaudíRSG fish at 0 dpa, before amputation (B, B′, B″) and after amputation (C, C′, C″). White arrows indicate labelled joint cells, yellow arrows indicate labelled segmental cells. D-D‴: Overview (D) and magnified confocal images of boxed region (D′-D‴) showing contribution of labelled col10a1 cells to segments (arrows, bone lining cells) and intersegmental joint regions (arrowheads) at 14 dpa (n = 14 fin rays, 5 fish). Asterisks indicate joints, dotted lines indicate the amputation plane. Scale bars = 100 μm. E: Strategy employed for col10a1 cell lineage tracing after osx cell ablation. F-G: Overview (F; scale bars = 200 μm) and magnified confocal images (G; scale bars = 50 μm) of re-emerged osx:mCherry-NTRo cells (F′, G′) and YFP labelled col10a1 cells (F″, G″) at 7 dpa showing contribution of labelled cells exclusively to joints (arrows). Dotted lines indicate the amputation plane. BF = brightfield. |
Reprinted from Developmental Biology, 455(1), Dasyani, M., Tan, W.H., Sundaram, S., Imangali, N., Centanin, L., Wittbrodt, J., Winkler, C., Lineage tracing of col10a1 cells identifies distinct progenitor populations for osteoblasts and joint cells in the regenerating fin of medaka (Oryzias latipes), 85-99, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.