Figure 4

Chemical compounds and PKIS inhibitors identified from zebrafish screen in two cystic cell culture models, MDCKII and Ox161c1. Cyst area of MDCKII (a) and Ox161c1 (b) cells after 17 and 14 days of compound exposure respectively to compounds identified in the zebrafish screen. Chemical classes as indicated, concentrations determined via prior dose response assays to exclude toxicity. Cyst area of MDCKII (c) and Ox161c1 (d) cells after 17 and 20 days of compound exposure respectively to compounds identified in the zebrafish screen compared to DMSO control exposed cells. PKIS inhibitors as indicated at 10 µM concentrations and 5 µM forskolin. Results represented by median +/− IQR. P values represented by * (****p ≤ 0.0001, ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05 and non-significant (ns): p > 0.05.). Median of DMSO baseline indicated with black line and significances via Kruskal-Wallis test with Dunn’s multiple comparison.