FIGURE

Fig. 5

ID

ZDB-FIG-200115-22

Publication

Leon et al., 2020 - Structural basis for adhesion G protein-coupled receptor Gpr126 function

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Fig. 5

The calcium-binding site is required for Gpr126 function in vivo.

a Surface conservation analysis (green, variable; purple, conserved) of CUB domain. The calcium-binding site is circled in magenta. D134 and F135 are indicated by arrows. b D134 and F135 were both mutated to alanines through homologous recombination of a 150 bp ssODN containing a 5 bp mutation (red nucleotides). c Genotyping assay for the gpr126^stl464 lesion. The 5 bp mutation introduces a BstUI restriction enzyme binding site. d 4 dpf wild-type larva compared to e 4 dpf gpr126^{stl464/stl464} larva with puffy ears (arrowheads). Scale bars (black) represent 100 μm. f 4 dpf wild-type larvae express mbp throughout the posterior lateral line nerve (PLLn, arrowhead), whereas g 4 dpf gpr126^{stl464/stl464} larva lack mbp expression along the PLLn (arrowhead). Scale bars (black) represent 100 μm. Asterisks indicate CNS.

Expression Data

Gene:	mbpa
Fish:	AB adgrg6^{stl464/stl464} (AB)
Anatomical Term:	posterior lateral line nerve
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adgrg6^{stl464/stl464} (AB)
Observed In:	inner ear posterior lateral line nerve
Stage:	Day 4

Phenotype Detail

Acknowledgments

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