FIGURE

Fig. 5

ID

ZDB-FIG-191230-811

Publication

Thestrup et al., 2019 - A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation

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Fig. 5

EphB/EphrinB signaling controls HPD lumen morphogenesis independently of other morphogenetic roles. a Conditional ephrinb1^EC expression is induced by heat-shock prior to or during CBD remodeling, at 50 hpf or 54 hpf, respectively. Embryos were analysed at 60 hpf (heat-shock at 50 hpf: n = 7, heat-shock at 54 hpf: n = 7, both N = 1). b–d Hnf4α (green), Prox1 (magenta) and aPKC (gray) labeling show that unlike in controls (b), conditional ephrinb1^EC expression at 50 hpf (c) and 54 hpf (d) leads to HPD, liver and pancreas morphology defects, ectopic Prox1-positive cells throughout the HPD (blue arrowheads), with a subset associated with ectopic lumina bifurcating from the main duct (outlined with blue dashed line) disrupted and disorganized HPD lumen (red arrowheads, c, d). Yellow dotted lines: liver, yellow dashed line: pancreas; blue arrow: HPD system, orange arrow: CBD, green arrowhead: HPA

Expression Data

Antibodies:	ab1-hnf4a Ab1-prkcz Ab5-prox1
Fish:	fci1Tg; nim25Tg
Condition:	heat shock
Anatomical Terms:	intestine liver and biliary system liver and biliary system digestive system duct pancreas
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fci1Tg; nim25Tg
Condition:	heat shock
Observed In:	common bile duct common bile duct duct epithelial cell common bile duct epithelial tube morphogenesis common bile duct lumen gall bladder hepatic duct liver and biliary system digestive system duct pancreas whole organism
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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