FIGURE

Figure 4.

ID

ZDB-FIG-191230-384

Publication

Cayuso et al., 2019 - Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness

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Figure 4.

Increased tension selectively restores boundary expression of <italic>rfng</italic> in <italic>epha4</italic> and <italic>ephrinb3</italic> mutants.

(A–K) Knockdown of mypt1 increases rfng expression at hindbrain boundaries in control embryos (F) and restores rfng expression at specific boundaries in ephrinb3^-/- (G), epha4^-/- (H), epha4^Δ651 (I) and epha4^KD (J) mutants compared to uninjected controls (A–E). (K) Percentage of embryos showing rfng expression at the different boundaries for: control (n = 38); MO-mypt1 (45); epha4a^-/- (13); epha4a^-/-MO-mypt1 (42); efnb3b^-/- (14); efnb3b^-/-MO-mypt1 (17); epha4a^Δ651 (15); epha4a^Δ651 MO-mypt1 (22); epha4a^KD (13); epha4a^KD MO-mypt1 (27). Dorsal views, anterior to the top. Arrowheads indicate normal boundary expression of rfng, while asterisks indicate reduction or absence of rfng expression at boundaries. Scale bar: 50 μm.

Expression Data

Gene:	rfng
Fish:	WT efnb3b^fci9/fci9 epha4a^fci5/fci5 epha4a^fci6/fci6 epha4a^fci7/fci7 WT + MO2-ppp1r12a efnb3b^fci9/fci9 + MO2-ppp1r12a epha4a^fci5/fci5 + MO2-ppp1r12a epha4a^fci6/fci6 + MO2-ppp1r12a epha4a^fci7/fci7 + MO2-ppp1r12a
Knockdown Reagent:	MO2-ppp1r12a
Anatomical Terms:	rhombomere 1 posterior margin rhombomere 2 posterior margin rhombomere 3 posterior margin rhombomere 4 posterior margin rhombomere 5 posterior margin rhombomere 6 posterior margin
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	efnb3b^fci9/fci9 epha4a^fci5/fci5 epha4a^fci6/fci6 epha4a^fci7/fci7 WT + MO2-ppp1r12a epha4a^fci5/fci5 + MO2-ppp1r12a epha4a^fci6/fci6 + MO2-ppp1r12a
Knockdown Reagent:	MO2-ppp1r12a
Observed In:	rhombomere 1 posterior margin rhombomere 2 posterior margin rhombomere 3 posterior margin rhombomere 4 posterior margin rhombomere 5 posterior margin rhombomere 6 posterior margin
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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