FIGURE

Fig. 1.

ID

ZDB-FIG-191230-300

Publication

Wang et al., 2019 - Heat resilience in embryonic zebrafish revealed by an in vivo stress granule reporter

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Fig. 1.

Establishment and characterization of GFP–G3BP1 knock-in zebrafish. (A) Schematic representation of the gene editing strategy used to insert GFP into the zebrafish g3bp1 locus. (B) Top, z-stacked picture showing the expression pattern of GFP–G3BP1 in 1 dpf embryo under basal conditions. Bottom, GFP reporter signal overlapped with bright-field image. Scale bars: 200 µm. (C) Stress granules in lens, retina and midbrain cells of 1 dpf GFP–G3BP1 knock-in zebrafish when exposed to 42°C for 0 or 10 min, and at 3 and 6 min after the removal of heat stress shock. Enlarged images of the areas in the midbrain region marked by yellow squares are shown in the lower panels. Scale bars: 10 µm.

Expression Data

Gene:	EGFP
Fish:	g3bp1^zf3186Tg
Condition:	heat shock
Anatomical Terms:	midbrain cytoplasmic stress granule optic cup cytoplasmic stress granule solid lens vesicle cytoplasmic stress granule
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.