FIGURE

Figure 2

ID

ZDB-FIG-191230-1883

Publication

Klingseisen et al., 2019 - Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Ultrastructural Analyses of the nfascb^ue56 Mutant Spinal Cord

(A and B) Transmission electron microscopy (TEM) images of sections through the spinal cord of wildtype (A) and nfascb^ue56 mutants (B) at 5 dpf. Dorsal to the top, midline left. Asterisks show myelinated cell bodies. Scale bars, 5 μm.

(C–D′) TEM images of cell bodies in wildtype (C and C′) and ue56 mutants (D and D′) showing enwrapment of a cell body in the mutant with multi-lammellar myelin. Scale bars, 1 μm.

(E–H) High magnification views of myelinated axons in the dorsal (E and F) and ventral (G and H) spinal cord of wildtype (E) and mutant (F) animals. Unmyelinated axons with a diameter >0.3 μm highlighted in blue. Scale bars, 0.5 μm.

(I) Number of axons with a diameter > 0.3 μm in wildtype and nfascb^ue56 mutants (wildtype mean 114.5 ± 23.71 SD, n = 6 animals, nfascb^ue56 mutant mean 111.4 ± 23.69 SD, n = 7 animals, p = 0.8201, t test).

(J) Total number of myelinated axons in wildtype and nfascb^ue56 mutants, and numbers in the dorsal and ventral domains of the spinal cord. (Total: wildtype mean 92.17 ± 19.57 SD and nfascb^ue56 mutant mean 53.57 ± 18.20 SD, p = 0.0036, t test. Dorsal: wildtype mean 36.17 ± 7.89 SD and nfascb^ue56 mutant mean 21.93 ± 8.60 SD, p = 0.0103, t test. Ventral: wildtype mean 56.00 ± 12.52 SD and nfascb^ue56 mutant mean 31.64 ± 11.81 SD, p = 0.0041, t test. All sets wildtype n = 6 animals and nfascb^ue56 mutant n = 7 animals).

(K) Average g-ratio per animal in wildtype and nfascb^ue56 mutants at 5 dpf (wildtype mean 0.72 ± 0.01 SEM, n = 6 animals and nfascb^ue56 mutant mean 0.73 ± 0.01 SEM, n = 7 animals, p = 0.7027, t test).

(L) G-ratios of all myelinated axons assessed in the dorsal spinal cord of wildtype (black) and nfascb^ue56 mutants (red) relative to axon caliber. Each point represents a myelinated axon.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nfascb^ue56/ue56
Observed In:	spinal cord spinal cord axon spinal cord central nervous system myelin formation spinal cord myelin sheath spinal cord neuronal cell body
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 51(6), Klingseisen, A., Ristoiu, A.M., Kegel, L., Sherman, D.L., Rubio-Brotons, M., Almeida, R.G., Koudelka, S., Benito-Kwiecinski, S.K., Poole, R.J., Brophy, P.J., Lyons, D.A., Oligodendrocyte Neurofascin Independently Regulates Both Myelin Targeting and Sheath Growth in the CNS, 730-744.e6, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell