FIGURE

Figure 5

ID

ZDB-FIG-191230-1587

Publication

Morishita et al., 2019 - A critical role of VMP1 in lipoprotein secretion

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Figure 5

Vmp1-deficient zebrafish and mice show accumulation of lipoproteins in the intestine, liver, and visceral endoderm. Immunohistochemistry of the intestine and liver from 6-dpf vmp1^+/- and vmp1^-/- zebrafish (A and C) and the visceral endoderm from 7.5-dpc Vmp1^gt/+ and Vmp1^gt/gt mice (B and D) using anti-SEC61B antibody (A and B), anti-APOB antibody (C and D), LipidTOX Red, and Hoechst33342. Arrows indicate the regions where the Sec61B/SEC61B signals were weak. The regions of zebrafish intestinal epithelial cells (E), intestinal lumen (L) or mouse visceral endoderm cells (VE) are shown as dashed lines. Data are representative of two independent experiments. Scale bars, 10 μm and 1 μm in the inset. The number of LipidTOX Red (+) structures with (black columns) or without (white columns) SEC61B (A and B) or APOB (C and D) per observed area was analyzed from at least two randomly selected areas using ImageJ software.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vmp1^jt3/jt3 (RW)
Observed In:	intestine lipoprotein liver lipoprotein
Stage:	Day 6

Phenotype Detail

Acknowledgments

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