FIGURE

Figure 3

ID

ZDB-FIG-191230-1377

Publication

Chen et al., 2019 - Loss of rps9 in Zebrafish Leads to p53-Dependent Anemia

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Figure 3

Deficiency in rps9 induces anemia in mutant embryos. (A-B) Detection of hemoglobin levels by o-dianisidine staining for rps9 in both siblings and mutants at 48 hpf and 3 dpf. (C-E, E’) Detection of erythroid cells by crossing rps9 mutant into LCR:EGFP transgenic fish line. At 24 hpf, the number of blood cells are reduced slightly; At 48 hpf and 3 dpf, erythrocytes are almost deprived in the trunk, and only a small amount of red blood cells remain in the cardiac region. (F) Wright-Giemsa staining of erythroid cells at 3 dpf in sibling and mutant.

Expression Data

Gene:	EGFP
Fish:	cz3325Tg (AB) rps9^la490/la490; cz3325Tg (AB) rps9^la491/la491; cz3325Tg (AB)
Anatomical Term:	nucleate erythrocyte
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rps9^la490/la490 (AB) rps9^la491/la491 (AB) rps9^la490/la490; cz3325Tg (AB) rps9^la491/la491; cz3325Tg (AB)
Observed In:	heart nucleate erythrocyte nucleate erythrocyte nucleate erythrocyte developmental maturation trunk hemoglobin trunk nucleate erythrocyte
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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