FIGURE

FIGURE 2

ID

ZDB-FIG-191230-1122

Publication

Wang et al., 2019 - Whole-Exome Sequencing Identifies Damaging de novo Variants in Anencephalic Cases

Other Figures

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FIGURE 2

Abnormal expression of wipi1 affected zebrafish embryo convergent extension. (A) Zebrafish embryos injected with wipi1-MO (antisense morpholino) show defects of convergent extension, including shortening of the trunk, and crooked or bent tails. To assess the extension defects quantitatively, the phenotypic classes were scored as grade 1 (normal), grade 2 (mild), grade 3 (moderate), and grade 4 (severe) in zebrafish embryos at 24 and 48 h post-fertilization (hpf) (scale bar = 500 μm). (B) Zebrafish embryos injected with wipi1-MO exhibit dosage-dependent convergent extension deficits at 24 and 48 hpf. Injection with wipi1-MO at dose of 2–6 ng showed a significant difference compared with control injection. (C) Overexpression of Wipi1 in zebrafish embryos at 24 and 48 hpf. Injection 200 pg mRNA and 300 pg mRNA resulted in a significant increase of malformed zebrafish. There was no correlation between mRNA dosage and severity of malformation (^∗P < 0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-wipi1
Knockdown Reagent:	MO1-wipi1
Observed In:	convergent extension post-vent region trunk whole organism
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.