FIGURE

Fig. 1

ID

ZDB-FIG-191018-14

Publication

Oikonomou et al., 2019 - The Serotonergic Raphe Promote Sleep in Zebrafish and Mice

Other Figures

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Fig. 1

tph2^−/− Zebrafish Are More Active and Sleep Less

(A) Maximum intensity projections of 5 days post-fertilization (dpf) tph2^+/− (left) or tph2^−/− (right) zebrafish brains immunostained for 5-HT. Middle: brain schematic. Top insets: 5-HT immunostaining of the pineal gland. Bottom insets: magnified view of the raphe. Scale bars, 50 μm. a, anterior; p, posterior; Pg, pineal gland; Pt, pretectal area; Hyp, hypothalamus; Ra, raphe; SRa, superior raphe; IRa, inferior raphe; MO, medulla oblongata.

(B–D) Activity (B), waking activity (C), and sleep (D) for tph2^+/+ (blue), tph2^+/− (cyan), and tph2^−/− (red) zebrafish. Line and shading represent mean ± SEM.

(E and F) Activity, waking activity, sleep, sleep bout number, sleep bout length during day (E) and night (F), and sleep latency at night (F).

n, number of animals. Not significant (ns) p > 0.05, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, Kruskal-Wallis test with Dunn’s multiple comparisons test.

Expression Data

Antibody:	Ab1-serotonin
Fish:	WT tph2^ct817/ct817
Anatomical Terms:	epiphysis hypothalamus inferior raphe nucleus medulla oblongata pretectum raphe nucleus superior raphe nucleus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tph2^ct817/ct817
Observed In:	epiphysis inferior raphe nucleus locomotory behavior medulla oblongata pretectum raphe nucleus sleep superior raphe nucleus
Stage Range:	Day 4 to Day 6

Phenotype Detail

Acknowledgments

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