Fig. 6-S1

(A–B₃) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing a EdU⁺/HuC/D⁺ neuron migrates to the upper region of injured optic tectum at 300 dpi. (B–B₃) The high-magnification images of boxed area in (A). White arrowheads indicate the EdU⁺/HuC/D⁺ neuron. Yellow dashed lines indicate the RG layer. White dashed lines and white asterisks indicate the stab wound that is not restored at 300 dpi. (C–D₃) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing the processes terminal of RG near the injury site are hypertrophic with high level of BLBP expression at 7 dpi. (D–D₃) The representative images of uninjured optic tectum. (E) Quantification of the area of stab wounds at 1–4 (1343 ± 315.7 μm²) and 300–400 dpi (1339 ± 768.6 μm²) showing that the stab wounds are not completely restored (mean ± SEM, ns, p>0.05; Wilcoxon test). (F–I₃) Representative images of EdU (red), HuC/D (green) and BLBP (gray) immunofluorescences showing stab wound that is surrounded by hypertrophic RG processes terminal (high level of BLBP expression, gray signals) is remained at 300 dpi. (H–H₃) are the representative images of uninjured optic tectum. (G–G₃) and (I–I₃) The high-magnification images of boxed areas in (F) and (H), respectively. (J and K) t-SNE and violin plots showing that hmgb2a and hmgb2b are enriched in proliferative-S and -G2 RG. (L–M₂) In situ hybridization showing PCNA⁺ RG (green cells) express hmgb2a (red signals) at 3 dpi. (M–M2) are the representative images of uninjured optic tectum. Yellow arrow heads indicate the injury site. RG, radial glia; TeO, tectal opticum; PGZ, periventricular gray zone; TS, torus semicircularis; Val, valvula cerebelli. Scale bars, 50 μm (A, F and H); 30 μm (B–B₃, C–C₃, D–D₃, G–G₃, I–I₃ and L–M₂).