FIGURE

Fig. 4

ID

ZDB-FIG-190820-27

Publication

Chan et al., 2019 - Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Transcriptional Competency of the Zygotic Genome Depends on the Translation of Maternal mRNAs

(A) Embryos treated with pateamine A (PatA) and cycloheximide (CHX) at 8- (1.25 hpf) and 32-cell stage (1.75 hpf) arrest zebrafish embryos at 16- and 64-cell stage, respectively.

(B and C) Biplot of Click-iT-seq RNA levels at 4 hpf comparing the level of transcription using intron signal. Embryos were treated with PatA+CHX at 8-cell stage (B) or at 32-cell stage (C), with and without α-amanitin. Dashed lines represent 4-fold change.

(D) Genome tracks representing normalized Click-iT-seq signal in the embryos described in (B) and (C). Click-iT-seq was normalized by the total number of mitochondrial RNAs as an internal control. RPM (STAR Methods).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 49, Chan, S.H., Tang, Y., Miao, L., Darwich-Codore, H., Vejnar, C.E., Beaudoin, J.D., Musaev, D., Fernandez, J.P., Benitez, M.D.J., Bazzini, A.A., Moreno-Mateos, M.A., Giraldez, A.J., Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation, 867-881.e8, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell