Fig. 2

Rap1b Is Involved in HE Specification

(A) Expression patterns of blood-flow-responsive gene klf2a in 36 hpf sibling (WT) and rap1b^ncv124 embryos.

(B and C) Expression patterns of arterial marker efnb2a in 24 hpf sibling (WT) and rap1b^ncv124 embryos (B) and in 24 hpf embryos injected with control MO or rap1b MO (C).

(D) Expression patterns of HE marker runx1 in 24 hpf sibling (WT) and rap1b^ncv124 embryos. Arrowheads indicate runx1-positive hemogenic ECs.

(E) Percentage of embryos showing normal (3 or more, blue) and decreased (less than 3, pink) numbers of runx1-positive hemogenic ECs in the AGM regions, as observed in (D). WT, n = 42; rap1b^ncv124, n = 43. ^∗∗∗p < 0.001.

(F) Expression patterns of runx1 in 26 hpf sibling (WT) and rap1b^ncv124embryos.

(G) Percentages of embryos showing normal (blue) and decreased (pink) expression of runx1 in the AGM regions, as observed in (F). WT, n = 12; rap1b^ncv124, n = 10. ^∗p < 0.001.

(H) Expression patterns of another HE marker, gata2b, in 24 hpf sibling (WT) and rap1b^ncv124 embryos.

In (A)–(D), (F), and (H), boxed areas are enlarged in the insets. Scale bars: 200 μm (A–D, F, and H)