Fig. 3

Primary culture of the basal keratinocytes from the reporter zebrafish expressing fluorescent proteins at larval stage. (A) Median fin fold of larva at 3 days post-fertilizations (dpf) expressing histone H2B-GFP which can visualize the nucleus and mCherry-CaaX in the basal keratinocytes. Basal keratinocytes were isolated from the median fin fold by the trypsin treatment and cultured in the L15 medium. (B) Time-lapse images of basal keratinocytes. White arrow indicates the direction of basal keratinocyte movement. (C) Basal keratinocytes can self-assemble and form colonies. The images on the top row show 8 basal keratinocytes which were separated one another after 2 days in culture. The images on the bottom row show a colony that consists of 8 basal keratinocytes at 3 days after culture. White dot lines show the shape of a cell or a colony. Scale bar is 50 μm (A–C).