FIGURE

Fig. 4

ID

ZDB-FIG-190815-26

Publication

Kang et al., 2019 - Ankrd45 Is a Novel Ankyrin Repeat Protein Required for Cell Proliferation

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Fig. 4

Knockdown of ANKRD45 results in cell cycle defects. (A,B) DIC (Differential Interference Contrast) still images obtained from time-lapse microscopy showing HeLa cells undergoing cell division. Compared with control siRNA-treated cells (A), knockdown of ANKRD45 (B) resulted in the delay of cell division and cell death. (C,D) Flow cytometry analysis of Annexin V-FITC and propidium iodide staining of the control and ANKRD45 siRNA-treated Hep3B cells. The percentage of apoptotic cells increased significantly after siRNA treatment (D). (E) Cell proliferation of Hep3B cells analyzed by CCK-8 assay. (F,G) Flow cytometry cell cycle analysis of Hep3B cells treated with control or siRNA. The percentage of cells in different phases of the cell cycle are shown in (G). (H) Confocal images showing proliferating and apoptotic Hep3B cells visualized by BrdU incorporation and the TUNEL assay. (I) Quantitative results of the percentage of BrdU positive cells in control or siRNA-treated Hep3B cells. (J) Quantitative results of the percentage of apoptotic cells from the TUNEL assay. NC, negative control. Scale bars: 25 μm. * p < 0.05, ** p < 0.01, *** p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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