FIGURE
Figure S4
Figure S4
CRISPR injection validation. (a) Cas9 only injected F0 fish have normal pigmentation at 120hpf while those injected with Cas9 and the slc45a5 guide RNAs are mostly devoid of pigment. (b) In control animals (GFAP:GFP) Pax2 is expressed in all MG by 120hpf. (c) F0 pax2a CRISPR injected animals lack Pax2 expression in most, but not all MG. (d) F1 pax2a CRISPR injected animals Pax2 is absent from all MG. (e) F1 CRISPR mutants with confirmed mutations have notably similar defects to those identified in F0 screen 3. (f) Percentages of animals with lethality and phenotypes after injections. Scale bars = 8μm. |
Expression Data
| Gene: | |
|---|---|
| Antibody: | |
| Fish: | |
| Knockdown Reagents: | |
| Anatomical Term: | |
| Stage: | Day 5 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Day 5 |
Phenotype Detail
Acknowledgments
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