FIGURE

Fig. 7.

ID

ZDB-FIG-190723-2564

Publication

Skvarca et al., 2019 - Enhancing acute kidney injury regeneration by promoting cellular dedifferentiation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7.

UPHD25 treatment efficacy requires intact RA signaling.Tg(PT:EGFP) transgenic zebrafish were used to analyze RTEC proliferation. (A-D) Immunofluorescence stain showing cells actively undergoing S-phase marked with PCNA antibody (red) and PT (green) in gent-AKI+DMSO (A), gent-AKI+Ro41 (B), gent-AKI+UPHD25 (C) and gent-AKI+Ro41+UPHD25 (D). PTs are outlined in white and arrows mark PCNA+ RTECs. (E) Quantification of PCNA+ RTECs in each treatment group. Mean_UPHD25=17.56 (N=34) vs Mean_Ro41+UPHD25=8.15 (N=34). Data pooled from three biological replicates are shown expressed as mean±s.e.m. One-way ANOVA: ****P<0.001. Scale bars: 20 μm.

Expression Data

Gene:	EGFP
Antibody:	Ab2-pcna
Fish:	nz4Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: retinoic acid receptor antagonist, chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin chemical treatment by environment: retinoic acid receptor antagonist, chemical treatment by injection: gentamycin
Anatomical Terms:	pronephric duct pronephric duct epithelial cell regenerating tissue pronephric duct
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz4Tg
Conditions:	chemical treatment by injection: gentamycin chemical treatment by environment: retinoic acid receptor antagonist, chemical treatment by injection: gentamycin chemical treatment by environment: EC 3.5.1.98 (histone deacetylase) inhibitor, chemical treatment by injection: gentamycin chemical treatment by environment: retinoic acid receptor antagonist, chemical treatment by injection: gentamycin
Observed In:	pronephric duct pronephric duct cell population proliferation regenerating tissue cell population proliferation regenerating tissue pronephric duct
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.