FIGURE

Fig 2

ID

ZDB-FIG-190723-1184

Publication

Desban et al., 2019 - Regulation of the apical extension morphogenesis tunes the mechanosensory response of microvilliated neurons

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Fig 2

The ring of actin colocalizes with the CSF-cN apical junctional complexes.

(A–C) Z-projections from lateral views of the spinal cord in 24-hpf embryos showing the colocalization of the ring of actin (LifeAct; arrowheads) with different markers of the AJCs: (A) Cdh2 for adherens junctions, (B) ZO-1 for tight junctions, and (C) Crb1 for the apical domain. (A) Double immunostaining for GFP and RFP in triple transgenic Tg(pkd2l1:Gal4,UAS:LifeAct-GFP;cryaa:V,cdh2:cdh2-RFP) embryos. (B, C) Double immunostaining for GFP and (B) ZO-1, or (C) Crb1 in Tg(pkd2l1:Gal4,UAS:LifeAct-GFP;cryaa:V) embryos. (D–F) Z-projections from lateral views of 72-hpf Tg(pkd2l1:Gal4,UAS:LifeAct-GFP;cryaa:V) larvae immunostained for GFP and (D) ZO-1, (E) Crb1, or (F) ZO-1 and Crb1. The markers ZO-1 and Crb1 are retained at the AJCs (arrowheads) in both V and DL CSF-cNs after differentiation. In DL cells, Crb1 expands to the apical extension. Scale bars, 10 μm. AJC, apical junctional complexes; Cdh2, Cadherin 2; Crb1, Crumbs 1; CSF-cN, cerebrospinal fluid-contacting neurons; DL, dorso-lateral; GFP, green fluorescent protein; hpf, hours post fertilization; RFP, red fluorescent protein; V, ventral; ZO-1, zonula-occludens-1.

Expression Data

Genes:	GFP TagRFP
Antibodies:	Ab1-tjp1 Ab3-crb1
Fish:	icm10Tg; icm28Tg icm10Tg; icm28Tg; zf518Tg
Anatomical Terms:	cerebrospinal fluid contacting neuron adherens junction cerebrospinal fluid contacting neuron apical junction complex cerebrospinal fluid contacting neuron apical part of cell cerebrospinal fluid contacting neuron cell projection cerebrospinal fluid contacting neuron tight junction
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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