FIGURE

Fig. 9

ID

ZDB-FIG-190716-40

Publication

Thomas et al., 2019 - Distinct progenitor populations mediate regeneration in the zebrafish lateral line

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Fig. 9

AP population doesn’t compensate for the loss of the DV population during hair cell regeneration.

(A–B) Maximum projections of tnfsf10l3:nlsEos; sost:NTR-GFP neuromasts following normal hair cell regeneration (A; Neo) or DV cell-ablated hair cell regeneration (B; Mtz3/Neo/Mtz5). Sost:NTR-GFP cells are shown in green, tnfsf10l3:nlsEos cells are shown in magenta, and anti-Parvalbumin-stained hair cells are shown in cyan. Arrowheads indicate nlsEos-positive hair cells. Scale bar = 10 μm. (C) Total number of hair cells per neuromast following hair cell regeneration. Neo: 10.36 ± 1.60, n = 50 neuromasts (10 fish); Mtz3/Neo/Mtz5: 7.98 ± 1.74, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001. (D) Number of nlsEos-positive hair cells per neuromast following hair cell regeneration. Neo: 2.88 ± 1.83, n = 50 neuromasts (10 fish); Mtz3/Neo/Mtz5: 3.14 ± 1.43, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.3855. (E) Percentage of hair cells per neuromast labeled by nlsEos following hair cell regeneration. Neo: 27.26 ± 16.00, n = 50 neuromasts (10 fish); Mtz3/Neo/Mtz5: 40.43 ± 19.44, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.0002.

Expression Data

Gene:	Eos
Fish:	sost^w216Tg; tnfsf10l3^w218Tg
Condition:	chemical ablation: neuromast support cell, chemical treatment by environment: neomycin
Anatomical Terms:	neuromast regenerating tissue neuromast hair cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sost^w216Tg; tnfsf10l3^w218Tg
Condition:	chemical ablation: neuromast support cell, chemical treatment by environment: neomycin
Observed In:	neuromast neuromast hair cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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