Partial knockdown of nup133 causes glomerular expansion in zebrafish. (a) Gross morphology of 3 dpf control and nup133 MO embryos (left panels: lateral view; middle panel: dorso-lateral view from two distinct embryos). Scale bar, 500 μm. Two-fold magnification of the indicated area is shown in the rightmost panels. Arrows indicate the pronephric cysts detected in the nup133 MO embryos. (b) Dorsal view of 3dpf Tg(wt1b:EGFP) embryos uninjected (control, top panels), injected with nup133 MO (middle panels), or sequentially injected with 3xHA-mCherry-Dr nup133 mRNA (wt mRNA) and nup133 MO (bottom panels). Overlays of transmission (gray) and GFP-signal (green) images reveal the glomerulus, proximal tubules, and exocrine pancreas. Scale bar, 500 μm. Two-fold magnification of the indicated area and of the same area from a distinct larvae are shown in the right and rightmost panels, respectively. The glomerular structure is indicated in brackets. Asterisks point to cystic dilations of the pronephros in nup133 MO (middle panels). Note that the nup133 MO + wt mRNA embryo displays a left-sided exocrine pancreas (arrowhead) (see also Supplemental Fig. S5). (c) Relative proportion of embryos showing or not kidney cysts at 3 dpf. For each condition, the total number of embryos analyzed is indicated (n=, quantified in 2 distinct experiments for control MO injections and 5 experiments for nup133 MO injections. See also Supplementary Fig. S3). Unlike the embryos injected with control MO, those injected with nup133 MO frequently feature kidney cysts. On the other hand nup133 MO + wt mRNA showed significantly fewer cysts than nup133 MO alone. ***P < 0.0001 using a Fisher exact probability test
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