Knockdown of Lgl2 in pen/lgl2 clutches leads to OLM and OPL abnormalities. Immunostaining (A–D′) and TEM (E–F′) of transverse retinal sections at 3 dpf of MO-injected pen/lgl2 clutches. (A–A″, C,C′) pen/lgl2 clutch injected with CNTR-MO and (B–B″,D,D′) pen/lgl2 clutch injected with ATG-MO1. ZO-1 (A,B), N-cadherin (A′,B′) and F-actin (C,D) localization to OLM (arrowheads) and OPL (arrows) is abnormal in morphants. Inset in B″ shows a projection of 15 optical planes in which discontinuity of the OLM is evident. Scale bars: 10 µm. (E,E′) pen/lgl2+/+ sibling injected with CNTR-MO shows a single, aligned layer of PRCs. E′ shows a higher magnification of a PRC adherens junction (arrowheads). (F,F′) pen/lgl2 mutant injected with ATG-MO1. PRCs have differentiated outer segments (green asterisks) but are disorganized. In the cells next to the disorganized area, normal adherens junctions are detected (F′, arrowheads). N, nucleus; M, mitochondria. Scale bar: 2 µm.
