FIGURE

Fig. 1

ID

ZDB-FIG-190703-2

Publication

Pandey et al., 2019 - A Smart Imaging Workflow for Organ-Specific Screening in a Cystic Kidney Zebrafish Disease Model

Other Figures

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Fig. 1

Automated imaging of an ift172-MO-based zebrafish model for human cystic kidney disease. (A–D) Dorsal views on pronephric areas in the Tg(wt1b:EGFP) embryos. Scale bar is 50 µm. (E–H) Bright-field stereo microscope images of the dose–response curve of ift172-MO on 72 hpf zebrafish larvae. Scale bar is 250 µm. (A,E) wild-type, (B,F) 50 µM ift172-MO, (C,G) 100 µM ift172-MO, and (D,H) 500 µM ift172-MO. (I,K) Overlay images of bright-field and fluorescence channels depicting wild-type and cystic pronephric phenotypes acquired with a 10x objective. Scale bar for I is 100 µm and J is 50 µm. (J,L) Enlarged fluorescent 10x views of I and K. (M,N) Montage images illustrating (M) overlay images or (N) pronephric areas of dorsally oriented larvae in a microtiter plate.

Expression Data

Gene:	EGFP
Fish:	li1Tg li1Tg + MO3-ift172
Knockdown Reagent:	MO3-ift172
Anatomical Term:	pronephros
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg + MO3-ift172
Knockdown Reagent:	MO3-ift172
Observed In:	caudal fin pericardium pronephros
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.