FIGURE

Fig. 2

ID

ZDB-FIG-190628-21

Publication

Giovannone et al., 2019 - Programmed conversion of hypertrophic chondrocytes into osteoblasts and marrow adipocytes within zebrafish bones

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Fig. 2

Vascularization of the Ch.

(A) Confocal projections of dissected Ch bones at five successive stages. Merged fluorescent and brightfield channels show the gradual replacement of the cartilage with a fat-filled core. col2a1a:mCherry-NTR highlights chondrocytes that become increasingly restricted to two growth plates (GP) at either end of the bone. fli1a:GFP labels endothelial cells and chondrocytes located in the central portions of the growth plates. Vascularization of the Ch increases over time. n = 2 at each stage. (B) Confocal projection shows networks of kdrl:GFP+ vascular endothelial and lyve1:DsRed+ lymphatic endothelial cells within an adult Ch bone. The inset shows a single confocal section through the boxed portion of the growth plate, with both blood and lymphatic vessels abutting the edges but not penetrating into the growth plate. n = 2. Scale bars = 100 μm (A) and 200 μm (B).

Expression Data

Genes:	DsRed EGFP mCherry
Fish:	nz101Tg; zf531Tg el559Tg; y48Tg
Anatomical Terms:	ceratohyal bone chondrocyte ceratohyal bone endothelial cell ceratohyal bone lymph vessel endothelium ceratohyal bone vascular endothelium
Stage Range:	Days 30-44 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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