Fig. 2

Effects of manipulation of food supplementation on hepatocarcinogensis and muscle wasting. 4-month-old male adult kras^G12V and WT zebrafish were treated with dox for 4 weeks and fed with different levels of artemia. A total of 5 mg artemia cysts/fish/day for normal feeding was given, defined as 100%. There were two underfeeding groups with 25% and 50% the amount of food of normal feeding, and two overfeeding groups with 200% and 300% the amount of normal feeding. In each feeding group, 20 fish were used to initiate the experiments. (A) Gross appearance and liver morphology based on liver-specific GFP expression in kras^G12V fish. (B) Survival curves. (C) Body weight excluding internal viscera. (D) H&E staining of liver sections of kras^G12V fish (left) and quantification (right). (E) IF staining of PCNA (red), Hnf4a (green) and DAPI (blue) in liver sections. Quantification of percentage of proliferating hepatocytes (right). (F) H&E staining of muscle sections of kras^G12V fish. Quantification of MFCSA of kras^G12V fish (right). *P<0.05. Scale bars: 2.5 mm in A; 10 μm in D-F.