FIGURE

Fig. 6-S1

ID

ZDB-FIG-190625-22

Publication

Yamaguchi et al., 2019 - zGrad is a nanobody-based degron system that inactivates proteins in zebrafish

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Fig. 6-S1

Tissue-specific Cxcr4b-EGFP degradation by zGrad.

(A) In situ hybridization against cxcr4b mRNA in a 38 hpf cxcr4b:zGrad; cxcr4b -/+ embryo. Arrow indicates the location of the primordium. Scale bar: 0.5 mm. (B) Images of live embryos of indicated genotypes stained for neuromasts with DASPEI at 4 dpf. Note that the cxcr4b:cxcr4b-EGFP-IRES-Kate2-CaaX-p1 transgene carries cryaa:dsRed, which expresses dsRED in the lens, and the cxcr4b:zGradtransgene carries myl7:mScarlet, which expresses mScarlet in the myocardium, as transgenic markers. Arrows indicate the position of the last neuromast. Scale bar: 1 mm. (C) Quantification of the position of the last neuromast normalized to body length of embryos shown in B. **=p < 0.01, N.S. = p > 0.05

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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