FIGURE

Fig. 6

ID

ZDB-FIG-190524-26

Publication

Rasouli et al., 2018 - The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling

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Fig. 6

Inhibition of Fgfr signaling can lead to cardiomyocyte extrusion in WT animals.

(A–D) Confocal images of 96 hpf hearts; WT animals treated with DMSO as a control or FGFR inhibitor (SU5402) from 75 to 96 hpf; maximum intensity projections of hearts in (A) and (D) are shown in (C) and (D) respectively; arrows point to extruding cardiomyocytes. (E–H) 75 hpf Tg(myl7: mCherry-CAAX) (E–F) or Tg(hsp70:dn-fgfr1-EGFP);Tg(myl7: mCherry-CAAX) (G–H) animals were heat-stressed at 39°C for 1 hr (F and H) and their hearts imaged at 96 hpf; arrow in (H) points to an extruding cardiomyocyte (n = 9/13 hearts). (I) klf2a^+/-; klf2b^-/- animals are more likely than WT siblings to exhibit cardiomyocyte extrusion upon Fgfr inhibition; number of treated larvae for each condition is shown above the individual columns. (J–K) Hearts of 96 hpf Tg(myl7: mCherry-CAAX); klf2 ^+/+ or klf2 ^-/- animals immunostained for pERK. (L–M) Hearts of 96 hpf Tg(fli1a:klf2b-p2A-tdTomato);Tg(myl7:EGFP-Hsa.HRAS); klf2 ^+/+ or klf2 ^-/- animals immunostained for pERK. Arrows and arrowheads point to extruding cardiomyocytes and pERK positive endocardial cells, respectively; V: ventricle, At: atrium; scale bars, 50 µm.

Expression Data

Genes:	EGFP mCherry
Antibody:	Ab9-mapk
Fish:	bns7Tg s883Tg bns7Tg; pd1Tg bns235Tg; s883Tg klf2a^bns11/bns11; klf2b^bns12/bns12; bns7Tg klf2a^bns11/bns11; klf2b^bns12/bns12; bns235Tg; s883Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Anatomical Terms:	atrial myocardium ventricular endocardium ventricular myocardium
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bns7Tg s883Tg bns7Tg; pd1Tg bns235Tg; s883Tg klf2a^bns11/+; klf2b^bns12/bns12; s883Tg klf2a^bns11/bns11; klf2b^bns12/bns12; bns7Tg klf2a^bns11/bns11; klf2b^bns12/bns12; bns235Tg; s883Tg
Conditions:	chemical treatment by environment: SU5402 heat shock
Observed In:	cardiac muscle cell compact layer of ventricle ventricular endocardium ventricular endocardium MAPK cascade ventricular myocardium ventricular cardiac muscle tissue morphogenesis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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