FIGURE

Fig. 2

ID

ZDB-FIG-190410-7

Publication

Meng et al., 2017 - The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors

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Fig. 2

Loss of stat4 leads to stenosis of PAAs 3–6.

(a) Genomic sequences of wild-type zebrafish, stat4 heterozygous mutants and homozygous mutants. The stat4 mutant has a two-base pair deletion, which causes a frame-shift mutation and a premature stop codon to form a truncated protein with 58 amino acids. (b,c) Pathological sections (10 μm per section) of wild-type and mutant embryos with Haematoxylin-Eosin staining at 72 hpf (n=12). (d,e) Confocal images of PAAs in the control Tg(kdrl:mCherry) embryos and the stat4−/−;Tg(kdrl:mCherry) line (n=9). (f,g) Images of PAAs 3–6 vessels trafficking blood flow in the control and stat4−/−;Tg(gata1:DsRed) embryos (n=9). (h,i) Images of PAAs trafficking blood flow in the control and the stat4−/−;Tg(fli1:EGFP);Tg(gata1:DsRed) line (n=8). (j–l) Control and the stat4−/−;Tg(fli1:nucEGFP) embryos show PAA endothelial cells (green) with cell number counts at 60 hpf (n=9). Error bars indicate s.d., unpaired two-tailed Student’s t-test, **P=0.003. (m) The model of PAAs 3–6 phenotype caused by stat4 deficiency is illustrated in the cartoon. Scale bars, 50 μm. LDA, lateral dorsal aorta; VA, ventral aorta; E, eye; H, heart. The brackets with asterisks highlight the malformed individual PAAs.

Expression Data

Genes:	DsRed EGFP mCherry
Fish:	y7Tg zf2071Tg zf2072Tg y1Tg; zf2071Tg stat4^{zf2070/zf2070}; y1Tg; zf2071Tg stat4^{zf2070/zf2070}; y7Tg stat4^{zf2070/zf2070}; zf2071Tg stat4^{zf2070/zf2070}; zf2072Tg
Anatomical Terms:	aortic arch blood cell aortic arch blood vessel endothelial cell blood vessel endothelial cell nucleus
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stat4^{zf2070/zf2070} stat4^{zf2070/zf2070}; y1Tg; zf2071Tg stat4^{zf2070/zf2070}; y7Tg stat4^{zf2070/zf2070}; zf2071Tg stat4^{zf2070/zf2070}; zf2072Tg
Observed In:	aortic arch vascular smooth muscle aortic arch 3 aortic arch 3 blood circulation aortic arch 3 blood vessel endothelial cell aortic arch 4 aortic arch 4 blood circulation aortic arch 4 blood vessel endothelial cell aortic arch 5 aortic arch 5 blood circulation aortic arch 5 blood vessel endothelium aortic arch 6 aortic arch 6 blood circulation aortic arch 6 blood vessel endothelial cell pharynx
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.