FIGURE

Fig. 1

ID

ZDB-FIG-190307-9

Publication

Hsieh et al., 2018 - Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

pparaa and pparab are expressed in the developing nervous system. A: real‐time polymerase chain reaction (RT‐PCR) analysis reveals that zebrafish pparaa and pparab transcripts are detected from 0.75 hpf to 10 hpf. pparaa displays relatively weak expression at 5.3, 8, and 10 hpf, and a high level of expression between 0.75 and 4.7 hpf, whereas pparab expression is detected at low levels at 8 and 10 hpf. Bottom bands show ef1α as a loading control. B: pparαa and pparαb expressions were analyzed using whole‐mount in situ hybridization. Abundant pparαa and pparαb expressions were observed in the embryo at 75% epiboly, bud, and 14‐somite stages. At 24 and 72 hpf, marked expressions were observed in the eyes (e), brain (b), spinal cord (sc), pharyngeal arches (pa), heart (h), and intestine (i). Staging of the embryos is shown in the bottom left corner of each panel. C: Embryos were sectioned sagittally through the neuroectoderm (i and iv) or transversely through the brain (ii, iii, v, and vi) after whole‐mount in situ hybridization. The regions of each section are indicated in (B).

Expression Data

Genes:	pparaa pparab
Fish:	TU
Anatomical Terms:	brain eye heart immature eye intestine pharyngeal arch spinal cord spinal cord neural tube whole organism
Stage Range:	2-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.