FIGURE

Fig. 1

ID

ZDB-FIG-190301-17

Publication

Wu et al., 2018 - Transgenic zebrafish models reveal distinct molecular mechanisms for cataract-linked αA-crystallin mutants

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Fig. 1

Transgenic expression of murine Cryaa mutants induced lens defects in 4dpf zebrafish embryos. (A) The lens-specific murine Cryaa transgenes displayed Cerulean marker in the heart. Upper Panel, DIC image. Lower panel, a white arrow marks the Cerulean marker in the heart. No overall morphology changes were observed in all transgenic carriers. (B) Representative DIC images of lenses from embryos expressing cataract-linked αA-crystallin mutants. The cataract phenotypes have been classified on the basis of the severity of defects. (i) WT, (ii, iii) minor defects (iv) major defects. (C) Embryos of lens-specific Rno.Cryaa transgenes (R49C and R116C mutations) displayed lens defects with various penetrance (scored at 4dpf). Removal of endogenous cryaa alleles exacerbated the lens defects. Error bars represented standard errors. Number of crosses is denoted in parentheses, n is the total number of screened embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vu615Tg vu616Tg cryaa^vu532/+; vu615Tg cryaa^vu532/+; vu616Tg cryaa^vu532/vu532; vu615Tg cryaa^vu532/vu532; vu616Tg
Observed In:	lens
Stage:	Day 4

Phenotype Detail

Acknowledgments

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