FIGURE

Fig. 7

ID

ZDB-FIG-190131-8

Publication

Gordon et al., 2018 - Hedgehog signaling regulates cell motility and optic fissure and stalk formation during vertebrate eye morphogenesis

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Fig. 7

Canonical signaling via Gli1 is required for coloboma in the ptch2^tc294z mutant. (A) Inhibition of non-canonical Hh signaling via Src-family kinases (SFKs) does not rescue coloboma in the ptch2^tc294z mutant. Graph shows percentage of ptch2^tc294z mutant embryos with coloboma, normalized to the DMSO control group. (B) Genetic loss of one or both copies of gli1 (using gli1^ts269) partially rescues the coloboma phenotype in the ptch2^tc294z mutant. Percentage of embryos with coloboma is shown. Numbers in parentheses indicate n. (C-F) Representative images of embryos from the genetic experiments shown in B, 55 hpf. (C) ptch2^+/−; gli1^any. (D) ptch2^−/−; gli1^+/+. (E) ptch2^−/−; gli1^+/−. (F) ptch2^−/−; gli1^−/−. Arrow indicates coloboma.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptch2^{tc294z/tc294z} gli1^ts269/+; ptch2^{tc294z/tc294z} gli1^ts269/ts269; ptch2^{tc294z/tc294z}
Observed In:	eye optic fissure
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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