Fig. 2

E2f4 is dispensable for MCC formation. A: Schematic showing CRISPR/Cas9-mediated genome editing at the zebrafish e2f4 locus using two guide RNAs targeting exon 1 and exon 6. After NHEJ, F1 carriers contained a 462-bp deletion between exon 1 and exon 6. This resulted in a 154-amino acid in-frame deletion. B: Kidney tubule of a 48 hpf wild-type embryo populated with clusters of MCCs (arrows). C: MCCs are unaffected in the kidney tubule of an e2f4 homozygous mutant (arrows). Acetylated-tubulin (Ace-tub; green), DAPI (blue). Scale bars: 10 µm. D: Kidney tubule of an e2f5 mutant embryo completely devoid of MCCs. E: Rescued MCCs in the kidney tubule of an e2f5 mutant by over-expression of E2f4 (arrows). Acetylated-tubulin (Ace-tub; green), DAPI (blue). Scale bars= 10 µm. F: MCCs around the lateral rim of a nasal placode (arrow) of a wild-type embryo at 72 hpf. G: Nasal placode MCCs are unaffected in an e2f4 mutant (arrow). Acetylated-tubulin (Ace-tub; green), γ-tubulin (γ-tub; red). L: lateral; M: medial. Scale bars = 10 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).